monoclonal anti cd169  (Bio-Rad)

Journal: Cell reports

Article Title: Medullary stromal cells define small intestinal lymph node identity in humans and mice

doi: 10.1016/j.celrep.2025.116441

Figure Lengend Snippet: (A and B) Representative flow cytometry plots (A) and average percentages (B) of LN macrophage subpopulations in duodenal (duo), jejunal (jej), ileal (ile), ceco-colonic (col), and inguinal (ing) LNs of 7-week-old C57BL/6 mice out of CD45 + (left) or total macrophages (right) ( n = 8). Data were pooled from two independent experiments. (C) Representative cross-section of murine duodenal LN (top), ceco-colonic LN (center), or mesenteric LN chain stained immunofluorescently with DAPI (dark blue), anti-CD169 (yellow), and anti-F4/80 (magenta). Scale bars, 400 μm. (D) Principal-component plot of RNA-seq data of actively translated mRNA pull-down from CD169 + cells in the indicated LNs ( n = 3 per LN type). (E) Heatmap and hierarchical clustering of DEGs (logFC > 1.5) between CD169 + cells from ileal and ceco-colonic LNs as determined by RNA-seq of actively translated mRNA. Symbols of transcripts measured by qPCR or pursued in bulk-sorted macrophage subsets (E) are in red. (F) Normalized CPM of indicated macrophage transcripts in sorted MSM, SSM, and MCM from the indicated LNs ( n = 3). (G) Normalized CPM of all DEGs between ileal and ceco-colonic LNs in sorted MSM, SSM, and MCM (left to right per cell type duo, jej, ile, col, and ing) as determined by RNA-seq. (H) Normalized CPM of the indicated macrophage gene in sorted MSM, SSM, and MCM from the indicated LNs ( n = 3). MSM, medullary sinus macrophage; SSM, subcapsular sinus macrophage; and MCM, medullary chord macrophage. Data are represented as mean ± SEM; ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by ANOVA.

Article Snippet: Rat monoclonal anti-mouse Cd169 , Bio-Rad , MCA884; RRID: AB_322416.

Techniques: Flow Cytometry, Staining, RNA Sequencing

Journal: eLife

Article Title: Ketogenic diet restrains aging-induced exacerbation of coronavirus infection in mice

doi: 10.7554/eLife.66522

Figure Lengend Snippet:

Article Snippet: Antibody , PE Rat monoclonal anti-CD169 , Thermo Fisher Scientific , Cat#12-5755-82; RRID: AB_2572625 , FACS (1:100) , .

Techniques: Isolation, Recombinant, cDNA Synthesis, SYBR Green Assay, Multiplex Assay, Software

Journal: eLife

Article Title: Dissecting the phenotypic and functional heterogeneity of mouse inflammatory osteoclasts by the expression of Cx3cr1

doi: 10.7554/eLife.54493

Figure Lengend Snippet: ( A ) Schematic representation of the differentiation of macrophages from the BM of Cx3cr1 GFP/+ mice. ( B ) FACS analysis of macrophage markers on BM-derived macrophages and ( C ) on GFP + (Cx3cr1 + ) and GFP neg (Cx3cr1 neg ) i-OCLs (n = 3). ( D ) Quantification of CD68 + CD169 + cells in Cx3cr1 + BM-derived macrophages and in Cx3cr1 neg and Cx3cr1 + i-OCL subsets. ( E ) Representative images of TRAcP staining on i-OCLs and BM-derived macrophages showing that contrasting to i-OCLs that all express TRAcP, BM-derived macrophages are negative for TRAcP expression. Scale bar = 50 µm.

Article Snippet: Antibody , Monoclonal anti-mouse CD169 , ThermoFisher Scientific , clone SER-4 , APC-conjugated (1:100).

Techniques: Derivative Assay, Staining, Expressing

Journal: eLife

Article Title: Dissecting the phenotypic and functional heterogeneity of mouse inflammatory osteoclasts by the expression of Cx3cr1

doi: 10.7554/eLife.54493

Figure Lengend Snippet:

Article Snippet: Antibody , Monoclonal anti-mouse CD169 , ThermoFisher Scientific , clone SER-4 , APC-conjugated (1:100).

Techniques:

Journal: eLife

Article Title: Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages

doi: 10.7554/eLife.52535

Figure Lengend Snippet: Z-stack of confocal microscopy images, showing Siglec-1 (red), microtubules (MT, green) and F-actin (grey) of day 6 HIV-1-infected macrophages, treated with cmMTB. Siglec-1 localizes on the thick MT + F-actin + TNT but not on thin MT - F-actin + TNT.

Article Snippet: Antibody , Mouse monoclonal anti-Siglec-1 (clone hsn 7D2) , Novus Biologicals , Cat# NB 600-534 RRID: AB_526814 , IF (10 μg/mL) IHC (1:200).

Techniques:

Journal: eLife

Article Title: Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages

doi: 10.7554/eLife.52535

Figure Lengend Snippet: 3D reconstitution of confocal images, showing Siglec-1 (red), HIV-1 Gag (green) and WGA (grey) of day 6 HIV-1-infected macrophages, treated with cmMTB.

Article Snippet: Antibody , Mouse monoclonal anti-Siglec-1 (clone hsn 7D2) , Novus Biologicals , Cat# NB 600-534 RRID: AB_526814 , IF (10 μg/mL) IHC (1:200).

Techniques:

Journal: eLife

Article Title: Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages

doi: 10.7554/eLife.52535

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-Siglec-1 (clone hsn 7D2) , Novus Biologicals , Cat# NB 600-534 RRID: AB_526814 , IF (10 μg/mL) IHC (1:200).

Techniques: Derivative Assay, Cell Culture, Transfection, Construct, Enzyme-linked Immunosorbent Assay, Blocking Assay, Control, Recombinant, Isolation, Western Blot, Fluorescence, Software, Imaging